Part:BBa_K2986014

Interleukin 10

IL-10 is a cytokine with multiple, pleiotropic, effects in immunoregulation and inflammation. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway.

Discovered in 1991 IL-10 was initially reported to suppress cytokine secretion, antigen presentation and CD4+ T cell activation. Further investigation has shown that IL-10 predominantly inhibits lipopolysaccharide (LPS) and bacterial product mediated induction of the pro-inflammatory cytokines TNFα, IL-1β, IL-12, and IFNγ secretion from Toll-Like Receptor (TLR) triggered myeloid lineage cells.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 126
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

MIT_MAHE 2020

Summary

IL-10 is a cytokine interleukin which has a central role in downregulating inflammatory cascades and matrix metalloproteinases, making it a likely candidate for use in therapeutic intervention to treat inflammation. It plays a role in limiting the host immune response so that the host does not harm its own cells and maintains normal tissue homeostasis. It is also responsible for enhancing B-cell survival, proliferation, and antibody secretion.

References

1.Steidler, L., Hans, W., Schotte, L., Neirynck, S., Obermeier, F., Falk, W., Fiers, W., & Remaut, E. (2000). Treatment of murine colitis by Lactococcus lactis secreting interleukin-10. Science (New York, N.Y.), 289(5483), 1352–1355. https://doi.org/10.1126/science.289.5483.1352

2. Yoon, S. I., Logsdon, N. J., Sheikh, F., Donnelly, R. P., & Walter, M. R. (2006). Conformational changes mediate interleukin-10 receptor 2 (IL-10R2) binding to IL-10 and assembly of the signaling complex. The Journal of biological chemistry, 281(46), 35088–35096. https://doi.org/10.1074/jbc.M606791200

3. Elenkov, I. J., & Chrousos, G. P. (1999). Stress Hormones, Th1/Th2 patterns, Pro/Anti-inflammatory Cytokines and Susceptibility to Disease. Trends in endocrinology and metabolism: TEM, 10(9), 359–368. https://doi.org/10.1016/s1043-2760(99)00188-5 "